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Journal: Medical Microbiology and Immunology
Article Title: A new bactericidal chlorinated derivative containing 2-aminooxazole potentiates antibacterial action of colistin against multidrug-resistant acinetobacter baumannii
doi: 10.1007/s00430-025-00854-y
Figure Lengend Snippet: A–D Results of macromolecular biosynthesis assay. Inhibition of biosynthetic pathway is indicated by lower incorporation of radioactively labeled precursors. A [ 3 H] N -acetylglucosamine (peptidoglycan synthesis), B [ 3 H] uridine (RNA synthesis), C [ 3 H] thymidine (DNA synthesis) and D [ 3 H] leucine (protein synthesis) in methicillin-resistant Staphylococcus aureus (ATCC 43300) strain, treated for 2 h with 4 × MIC of VAN, RIF, CIP, CHL, CHX, and AB15. Results are expressed as the percentage of biomolecule incorporation related to untreated controls. The values shown are means of two independent experiments prepared in duplicates ± SEM. Significant reduction in biosynthetic pathway compared to control is indicated by p -value, where p < 0.05 was accepted as statistically significant (* p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001), determined by nonparametric one-way (ANOVA) test (Kruskal–Wallis test)
Article Snippet: As shown in Fig. , the action of AB15 resulted in a statistically significant decrease in the incorporation of radiolabeled precursors participating in the protein synthesis pathway (Fig. D) of the
Techniques: Inhibition, Labeling, DNA Synthesis, Control
Journal: Medical Microbiology and Immunology
Article Title: A new bactericidal chlorinated derivative containing 2-aminooxazole potentiates antibacterial action of colistin against multidrug-resistant acinetobacter baumannii
doi: 10.1007/s00430-025-00854-y
Figure Lengend Snippet: Membrane depolarization assay. Membrane potential was monitored using the fluorescence dye, DiSC3(5), (λ Ex = 620 nm, λ Em = 680 nm). Methicillin-resistant Staphylococcus aureus (ATCC 43300) was stained with 0.5 mM DiSC3(5). After 10 min of measurement (black arrow), AB15, positive control represented by chlorhexidine (CHX), in final concentrations corresponding to 4 × MIC were added. The graph depicts the mean of six replicates and the standard deviation
Article Snippet: As shown in Fig. , the action of AB15 resulted in a statistically significant decrease in the incorporation of radiolabeled precursors participating in the protein synthesis pathway (Fig. D) of the
Techniques: Membrane, Fluorescence, Staining, Positive Control, Standard Deviation